Flow cytometry is a powerful technology that enables identification of cells or generally any particle in suspension on the basis of their physical and/or fluorescent characteristics.
The Becton-Dickinson FACSAriaIIITM Cell Sorter allows to identify individual classes and populations of cells and measure their surface and intracellular markers to estimate functional states of cells, to perform cell cycle analysis, to study apoptosis, phagocytosis, kinetics of cell processes, to perform molecular-genetic analyses using fluorescence probes, etc.
It is also possible to extend flow cytometry to the collection into a tube, plates or onto slides of subsets of a mixed cell population, in a purified form, by the electrostatic deflection of droplets containing target cells away from the main flow stream.
LOCATION
Building C, Largo Meneghetti 2; Cod. Geotec 00200 -1 010
STAFF AND CONTACT LOCATION
Scientific supervisor
Prof. Lisa Dalla Via
Facility manager
Dr. Federico Cusinato
Staff
Dr. Federico Cusinato
Dr. Erica Gentilin
049 8275778/5082
federico.cusinato@unipd.it
9:00 am – 5:00 pm Monday-Friday
HOW TO BOOK
Ask the staff for booking. Time of slot 30 min. Instructions are provided to users on the methodologies and the applications. The maintenance and operation of the equipment and the set-up of methods of analysis are coordinated by the staff in charge. Another task is the supervision of the planning, booking and reporting of devices.
The analyses are carried out by the staff, in the presence of the users, while others are directly done by the expert users, after specific training by the staff (Certificate of attendance). The staff provides assistance and support in teaching duties and to external users.
The use of the instrument, supported by the staff, is also allowed to some “super-users”, experts in some specific fields, who can make their contribution to scientific research in terms of innovation in the development of new methods and new applications.
EQUIPMENT
Becton-Dickinson FACSAriaIIITM Cell Sorter
The BD FACSAriaTM III flow cytometer is a high-speed fixed-alignment benchtop cell sorter. It is at the same time cell analyzer and cell sorter.
The sample can be acquired in a variety of tubes, from 15-mL centrifuge tube to 1.0-mL microtube. The temperature of sample injection chamber as well as the agitation settings can be controlled by BD FACSDiva software. Being equipped with four lasers, it provides multicolor analysis of distinct types of separated cells or particles with the detection of up to 16 fluorescent markers and 2 scatter parameters at a time. As cell sorter is provided with different nozzles allowing to sort a wide range of particle.
It is able to physically separate up to 4 cell populations simultaneously and collect them into tubes, plates, or slides.
PUBLICATIONS
Some publications produced utilizing the facility
Baggio C, Ramaschi GE, Oliviero F, Ramonda R, Sfriso P, Trevisi L, Cignarella A, Bolego C. Sex-dependent PD-L1/sPD-L1 trafficking in human endothelial cells in response to inflammatory cytokines and VEGF. Biomed Pharmacother. 2023 Jun;162:114670. doi: 10.1016/j.biopha.2023.114670.
Colombo E, Coppini DA, Polito L, Ciriello U, Paladino G, Hyeraci M, Di Paolo ML, Nordio G, Dalla Via L, Passarella D. Cannabidiol as Self-Assembly Inducer for Anticancer Drug-Based Nanoparticles. Molecules. 2022 Dec 23;28(1):112. doi: 10.3390/molecules28010112.
Facchetti G, Christodoulou MS, Mendoza LB, Cusinato F, Dalla Via L, Rimoldi I. Biological Properties of New Chiral 2-Methyl-5,6,7,8-tetrahydroquinolin-8-amine-based Compounds. Molecules. 2020 Nov 27;25(23):5561. doi: 10.3390/molecules25235561.
