GAS AND LIQUID CHROMATOGRAPHY FACILITY
The Gas and Liquid Chromatography Facility allows analytical, preparative and semi-preparative separations of components from a mixture. The facility groups instruments based on chromatographic techniques: HPLC-UV, HPLC-DAD, FPLC, FLASH-Chromatography, preparative HPLC and GC-FID.
Applications
- Identification and purification of small organic molecules;
- Identification and quantification of compounds in complex mixtures;
- Separation and purification of peptides, proteins, nucleic acids and other biomolecules.
General Info
Instructions are provided to users on the methodologies and the applications. The maintenance and operation of the equipment and the set-up of methods of analysis are coordinated by the staff in charge, as well as the supervision of the planning, booking and reporting of devices.
Location
DSF – Edificio A (GeoTec 00190 -1 063)
Facility manager
Susanna Vogliardi, PhD.
Scientific Supervisor
Gianfranco Pasut, Prof.
Staff
Dr. Susanna Vogliardi, PhD; Dr. Sara Bersani, Dr. Luca Carraretto, Dr. Rossano Chinchio, Dr. Riccardo Lorenzin.
Phone: 049 8275779, e-mails: susanna.vogliardi@unipd.it; sara.bersani@unipd.it; luca.carraretto@unipd.it; riccardo.lorenzin@unipd.it; rossano.chinchio@unipd.it
9:00 a.m. – 5:00 p.m. Monday-Friday
Contact the staff before booking, asking about facility rates.
Booking Information
Ask the staff for booking . Time slots of 30 min are available.
Expert users, after a specific training carried out by a staff member or another expert user (Certificate of attendance), can book (GC-LC Facility) and use the instruments by their own, while, if needed, the analysis could be carried out by the staff, in the presence of the users. The staff aids and support in teaching duties and to external users.
Equipment
HPLC Varian ProStar
HPLC PuriFlash 5.125
Automated Flash Chromatography
GC Agilent 6890 FID
The Gas and Liquid Chromatography Facility is equipped with a GC Agilent 6890-FID instrument. Gas chromatography (GC) allows to separate and quantify volatile components, vaporized by heat, of a mixture. It is assembled with a split/splitless injector and Electronic Pneumatic Control (EPC) system which allows control of flows for FID ignition directly from the GC or via remote control. Flame ionization detection (FID) is the most commonly used detector in gas chromatographs due to its high stability, sensitivity and linearity for measuring organic substances.
GC Varian 3800-FID
The Gas and Liquid Chromatography Facility is equipped with a GC Vaian 3800-FID instrument. Gas chromatography (GC) allows to separate and quantify volatile components, vaporized by heat, of a mixture. It is assembled with a split/splitless injector and manual pneumatics flow control for FID ignition. Flame ionization detection (FID) is the most commonly used detector in gas chromatographs due to its high stability, sensitivity and linearity for measuring organic substances.
GC Varian CP 3800 with
Ion Trap Saturn 2000
The Gas and Liquid Chromatography Facility is equipped with a GC Varian CP 3800 with Ion Trap Saturn 2000 mass analyzer. Gas chromatography (GC) allows to separate and quantify components, vaporized by heat, of a mixture. It is assembled with a split/splitless injector and Electronic Pneumatic Control (EPC) system which allows control of flows for FID ignition directly from the GC or via remote control. The instrument is associated with an ion trap mass analyzer with an Electron Impact ionization system. This function makes it possible to identify analytes based on their fragments.
